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pcmv vsv g envelope vectors  (Addgene inc)


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    Structured Review

    Addgene inc pcmv vsv g envelope vectors
    Pcmv Vsv G Envelope Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 3091 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/envelope+vector+pcmv+vsv+g/pCMV-VSV-G+(Plasmid+%238454)/pmc12701018-32-14-17
    Average 96 stars, based on 3091 article reviews
    pcmv vsv g envelope vectors - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Centrosome linker diversity and its function in centrosome clustering and mitotic spindle formation.
    Article Snippet: The centrosome linker joins the two interphase centrosomes of a cell into one microtubule organizing center.. Despite increasing knowledge on linker components, linker diversity in different cell types and their role in cells with supernumerary centrosomes remained unexplored.. Here, we identified Ninein as a C-Nap1anchored centrosome linker component that provides linker function in RPE1 cells while in HCT116 and U2OS cells, Ninein and Rootletin link centrosomes together.

    Article Title: ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling.
    Article Snippet: Luciferase and cell viabilitymeasurementswere performedusing anOrion II microplate Luminometer and the Simplicity software (Berthold), SEAP activity was measured at 650nm by using a Vmax kinetic microplate reader (Molecular Devices) and the SoftMax Pro 7.0.3 software. .. Generation of U2OS cells stably expressing STING and mtDNA depletion The pMSCV-hygro plasmid carrying WT STING1 cDNA (Addgene plasmid #102598) or empty vector were used in combination with packaging vector pCL-Ampho (Novus) and envelope vector pCMV-VSV-G (Addgene plasmid #8454) to produce retroviral vectors as described for lentiviral vectors. .. 100,000U2OS cells were transducedwith 0.5mL retroviral vectors, 8μg/mL polybrene (Millipore, TR-1003-G) and 10mM HEPES (Invitrogen, H3375) in 12-well plates, and medium replaced 24h later.

    Article Title: Enhanced cGAS-STING–dependent interferon signaling associated with mutations in ATAD3A
    Article Snippet: .. The pMSCV-hygro plasmid carrying WT STING1 cDNA (#102598; Addgene) was used in combination with packaging vector pCL-Ampho (Novus) and envelope vector pCMV-VSV-G (plasmid #8454; Addgene) to produce retroviral vectors as described for shRNA lentiviral vectors. .. 100,000 U2OS cells were transduced with 0.5 ml retroviral vectors, 8 μg/ml polybrene (Millipore), and 10 mM Hepes (Invitrogen) in 12-well plates and medium replaced 24 h later.

    Article Title: The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication
    Article Snippet: .. For the production of viral particles, the gene of interest was cloned into pRetroX-TRE3G (Clontech) and co-transfected with the envelope vector pCMV-VSV-G (Addgene) to a HEK293-based retroviral packaging cell line (GP2-293, Clontech). .. Media were changed 24 h after transfection, and the virus-containing media was harvested 48 h after transfection and filtered using a 0.45 μM filter (Millipore).

    Article Title: E4BP4 in macrophages induces an anti-inflammatory phenotype that ameliorates the severity of colitis
    Article Snippet: Plasmid 24 vectors were transformed into Stellar Competent Cells (Takara Bio #636766) and then purified 25 using the QIAfilter Plasmid Midi Kit (QIAGEN #12243). .. CRISPR plasmids were packaged in 26 HEK293TN cells via transfection with the envelope vector pCMV-VSV-G (a gift from Bob 27 Weinberg via Addgene plasmid # 8454) and packaging vector pCMV delta R8.2 (a gift from Didier 28 Trono via Addgene #12263) using Lipofectamine LTX and Plus Reagent (Thermo Fisher 29 Scientific #15338-100). .. Viral supernatant was then harvested and concentrated using the Lenti-30 X Concentrator (Takara Bio).

    Article Title: Enhanced cGAS-STING-dependent interferon signaling associated with mutations in ATAD3A
    Article Snippet: .. The pMSCV-hygro plasmid carrying WT STING1 cDNA (Addgene #102598) was used in combination with packaging vector pCL-Ampho (Novus) and envelope vector pCMV-VSV-G (Addgene plasmid #8454) to produce retroviral vectors as described for shRNA lentiviral vectors. .. 100,000 U2OS cells were transduced with 0.5 mL retroviral vectors, 8 μg/mL polybrene (Millipore) and 10 mM HEPES (Invitrogen) in 12-well plates and medium replaced 24 hours later.

    Article Title: The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication.
    Article Snippet: .. For the production of viral particles, the gene of interest was cloned into pRetroX-TRE3G (Clontech) and co-transfected with the envelope vector pCMV-VSV-G (Addgene) to a HEK293-based retroviral packaging cell line (GP2-293, Clontech). .. Media were changed 24 h after transfection, and the viruscontaining media was harvested 48h after transfection and filtered using a 0.45 μM filter (Millipore).

    Article Title: ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling
    Article Snippet: Luciferase and cell viability measurements were performed using an Orion II microplate Luminometer and the Simplicity software (Berthold), SEAP activity was measured at 650 nm by using a Vmax kinetic microplate reader (Molecular Devices) and the SoftMax Pro 7.0.3 software. .. The pMSCV-hygro plasmid carrying WT STING1 cDNA (Addgene plasmid #102598) or empty vector were used in combination with packaging vector pCL-Ampho (Novus) and envelope vector pCMV-VSV-G (Addgene plasmid #8454) to produce retroviral vectors as described for lentiviral vectors. .. 100,000 U2OS cells were transduced with 0.5 mL retroviral vectors, 8 μg/mL polybrene (Millipore, TR-1003-G) and 10 mM HEPES (Invitrogen, H3375) in 12-well plates, and medium replaced 24 h later.

    Generated:

    Article Title: Centrosome linker diversity and its function in centrosome clustering and mitotic spindle formation.
    Article Snippet: The centrosome linker joins the two interphase centrosomes of a cell into one microtubule organizing center.. Despite increasing knowledge on linker components, linker diversity in different cell types and their role in cells with supernumerary centrosomes remained unexplored.. Here, we identified Ninein as a C-Nap1anchored centrosome linker component that provides linker function in RPE1 cells while in HCT116 and U2OS cells, Ninein and Rootletin link centrosomes together.

    Stable Transfection:

    Article Title: ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling.
    Article Snippet: Luciferase and cell viabilitymeasurementswere performedusing anOrion II microplate Luminometer and the Simplicity software (Berthold), SEAP activity was measured at 650nm by using a Vmax kinetic microplate reader (Molecular Devices) and the SoftMax Pro 7.0.3 software. .. Generation of U2OS cells stably expressing STING and mtDNA depletion The pMSCV-hygro plasmid carrying WT STING1 cDNA (Addgene plasmid #102598) or empty vector were used in combination with packaging vector pCL-Ampho (Novus) and envelope vector pCMV-VSV-G (Addgene plasmid #8454) to produce retroviral vectors as described for lentiviral vectors. .. 100,000U2OS cells were transducedwith 0.5mL retroviral vectors, 8μg/mL polybrene (Millipore, TR-1003-G) and 10mM HEPES (Invitrogen, H3375) in 12-well plates, and medium replaced 24h later.

    Expressing:

    Article Title: ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling.
    Article Snippet: Luciferase and cell viabilitymeasurementswere performedusing anOrion II microplate Luminometer and the Simplicity software (Berthold), SEAP activity was measured at 650nm by using a Vmax kinetic microplate reader (Molecular Devices) and the SoftMax Pro 7.0.3 software. .. Generation of U2OS cells stably expressing STING and mtDNA depletion The pMSCV-hygro plasmid carrying WT STING1 cDNA (Addgene plasmid #102598) or empty vector were used in combination with packaging vector pCL-Ampho (Novus) and envelope vector pCMV-VSV-G (Addgene plasmid #8454) to produce retroviral vectors as described for lentiviral vectors. .. 100,000U2OS cells were transducedwith 0.5mL retroviral vectors, 8μg/mL polybrene (Millipore, TR-1003-G) and 10mM HEPES (Invitrogen, H3375) in 12-well plates, and medium replaced 24h later.

    Retroviral:

    Article Title: ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling.
    Article Snippet: Luciferase and cell viabilitymeasurementswere performedusing anOrion II microplate Luminometer and the Simplicity software (Berthold), SEAP activity was measured at 650nm by using a Vmax kinetic microplate reader (Molecular Devices) and the SoftMax Pro 7.0.3 software. .. Generation of U2OS cells stably expressing STING and mtDNA depletion The pMSCV-hygro plasmid carrying WT STING1 cDNA (Addgene plasmid #102598) or empty vector were used in combination with packaging vector pCL-Ampho (Novus) and envelope vector pCMV-VSV-G (Addgene plasmid #8454) to produce retroviral vectors as described for lentiviral vectors. .. 100,000U2OS cells were transducedwith 0.5mL retroviral vectors, 8μg/mL polybrene (Millipore, TR-1003-G) and 10mM HEPES (Invitrogen, H3375) in 12-well plates, and medium replaced 24h later.

    Article Title: Enhanced cGAS-STING–dependent interferon signaling associated with mutations in ATAD3A
    Article Snippet: .. The pMSCV-hygro plasmid carrying WT STING1 cDNA (#102598; Addgene) was used in combination with packaging vector pCL-Ampho (Novus) and envelope vector pCMV-VSV-G (plasmid #8454; Addgene) to produce retroviral vectors as described for shRNA lentiviral vectors. .. 100,000 U2OS cells were transduced with 0.5 ml retroviral vectors, 8 μg/ml polybrene (Millipore), and 10 mM Hepes (Invitrogen) in 12-well plates and medium replaced 24 h later.

    Article Title: The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication
    Article Snippet: .. For the production of viral particles, the gene of interest was cloned into pRetroX-TRE3G (Clontech) and co-transfected with the envelope vector pCMV-VSV-G (Addgene) to a HEK293-based retroviral packaging cell line (GP2-293, Clontech). .. Media were changed 24 h after transfection, and the virus-containing media was harvested 48 h after transfection and filtered using a 0.45 μM filter (Millipore).

    Article Title: Enhanced cGAS-STING-dependent interferon signaling associated with mutations in ATAD3A
    Article Snippet: .. The pMSCV-hygro plasmid carrying WT STING1 cDNA (Addgene #102598) was used in combination with packaging vector pCL-Ampho (Novus) and envelope vector pCMV-VSV-G (Addgene plasmid #8454) to produce retroviral vectors as described for shRNA lentiviral vectors. .. 100,000 U2OS cells were transduced with 0.5 mL retroviral vectors, 8 μg/mL polybrene (Millipore) and 10 mM HEPES (Invitrogen) in 12-well plates and medium replaced 24 hours later.

    Article Title: The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication.
    Article Snippet: .. For the production of viral particles, the gene of interest was cloned into pRetroX-TRE3G (Clontech) and co-transfected with the envelope vector pCMV-VSV-G (Addgene) to a HEK293-based retroviral packaging cell line (GP2-293, Clontech). .. Media were changed 24 h after transfection, and the viruscontaining media was harvested 48h after transfection and filtered using a 0.45 μM filter (Millipore).

    Article Title: ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling
    Article Snippet: Luciferase and cell viability measurements were performed using an Orion II microplate Luminometer and the Simplicity software (Berthold), SEAP activity was measured at 650 nm by using a Vmax kinetic microplate reader (Molecular Devices) and the SoftMax Pro 7.0.3 software. .. The pMSCV-hygro plasmid carrying WT STING1 cDNA (Addgene plasmid #102598) or empty vector were used in combination with packaging vector pCL-Ampho (Novus) and envelope vector pCMV-VSV-G (Addgene plasmid #8454) to produce retroviral vectors as described for lentiviral vectors. .. 100,000 U2OS cells were transduced with 0.5 mL retroviral vectors, 8 μg/mL polybrene (Millipore, TR-1003-G) and 10 mM HEPES (Invitrogen, H3375) in 12-well plates, and medium replaced 24 h later.

    shRNA:

    Article Title: Enhanced cGAS-STING–dependent interferon signaling associated with mutations in ATAD3A
    Article Snippet: .. The pMSCV-hygro plasmid carrying WT STING1 cDNA (#102598; Addgene) was used in combination with packaging vector pCL-Ampho (Novus) and envelope vector pCMV-VSV-G (plasmid #8454; Addgene) to produce retroviral vectors as described for shRNA lentiviral vectors. .. 100,000 U2OS cells were transduced with 0.5 ml retroviral vectors, 8 μg/ml polybrene (Millipore), and 10 mM Hepes (Invitrogen) in 12-well plates and medium replaced 24 h later.

    Article Title: Enhanced cGAS-STING-dependent interferon signaling associated with mutations in ATAD3A
    Article Snippet: .. The pMSCV-hygro plasmid carrying WT STING1 cDNA (Addgene #102598) was used in combination with packaging vector pCL-Ampho (Novus) and envelope vector pCMV-VSV-G (Addgene plasmid #8454) to produce retroviral vectors as described for shRNA lentiviral vectors. .. 100,000 U2OS cells were transduced with 0.5 mL retroviral vectors, 8 μg/mL polybrene (Millipore) and 10 mM HEPES (Invitrogen) in 12-well plates and medium replaced 24 hours later.

    Clone Assay:

    Article Title: The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication
    Article Snippet: .. For the production of viral particles, the gene of interest was cloned into pRetroX-TRE3G (Clontech) and co-transfected with the envelope vector pCMV-VSV-G (Addgene) to a HEK293-based retroviral packaging cell line (GP2-293, Clontech). .. Media were changed 24 h after transfection, and the virus-containing media was harvested 48 h after transfection and filtered using a 0.45 μM filter (Millipore).

    Article Title: The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication.
    Article Snippet: .. For the production of viral particles, the gene of interest was cloned into pRetroX-TRE3G (Clontech) and co-transfected with the envelope vector pCMV-VSV-G (Addgene) to a HEK293-based retroviral packaging cell line (GP2-293, Clontech). .. Media were changed 24 h after transfection, and the viruscontaining media was harvested 48h after transfection and filtered using a 0.45 μM filter (Millipore).

    CRISPR:

    Article Title: E4BP4 in macrophages induces an anti-inflammatory phenotype that ameliorates the severity of colitis
    Article Snippet: Plasmid 24 vectors were transformed into Stellar Competent Cells (Takara Bio #636766) and then purified 25 using the QIAfilter Plasmid Midi Kit (QIAGEN #12243). .. CRISPR plasmids were packaged in 26 HEK293TN cells via transfection with the envelope vector pCMV-VSV-G (a gift from Bob 27 Weinberg via Addgene plasmid # 8454) and packaging vector pCMV delta R8.2 (a gift from Didier 28 Trono via Addgene #12263) using Lipofectamine LTX and Plus Reagent (Thermo Fisher 29 Scientific #15338-100). .. Viral supernatant was then harvested and concentrated using the Lenti-30 X Concentrator (Takara Bio).

    Transfection:

    Article Title: E4BP4 in macrophages induces an anti-inflammatory phenotype that ameliorates the severity of colitis
    Article Snippet: Plasmid 24 vectors were transformed into Stellar Competent Cells (Takara Bio #636766) and then purified 25 using the QIAfilter Plasmid Midi Kit (QIAGEN #12243). .. CRISPR plasmids were packaged in 26 HEK293TN cells via transfection with the envelope vector pCMV-VSV-G (a gift from Bob 27 Weinberg via Addgene plasmid # 8454) and packaging vector pCMV delta R8.2 (a gift from Didier 28 Trono via Addgene #12263) using Lipofectamine LTX and Plus Reagent (Thermo Fisher 29 Scientific #15338-100). .. Viral supernatant was then harvested and concentrated using the Lenti-30 X Concentrator (Takara Bio).



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